39 4d5 Search Results


isl1  (Developmental Studies Hybridoma Bank)
97
Developmental Studies Hybridoma Bank isl1
Fig. 1. LIM homeobox 1 (Lhx1) is expressed in the pancreas. A: embryonic day (E)14.5 Lhx1-LacZ reporter mouse pancreas tissue staining demonstrates an active Lhx1 locus in the developing pancreas. Tissues were stained with X-gal (blue) and eosin (pink). Bottom: 40 magnification of E14.5 Lhx1-LacZ reporter activity in mouse pancreas, highlighted by black arrowheads B: immunofluorescence from 5-mo-old adult mouse pancreas tissue demonstrating Lhx1 (red) in insulin (green) co-expressing cells. C: co-immunofluorescence with 5-mo-old wild-type mouse pancreata indicates that Lhx1 (blue), <t>Islet-1</t> <t>(Isl1;</t> red), and LIM domain-binding protein 1 co-regulator (Ldb1; green) are co-expressed in islets. D: Lhx1 and Isl1 expression relative to Gapdh was assessed with TC3, Min6, and adult mouse islet cDNA (top); Western blotting analysis of relative Lhx1 protein levels in TC3, Min6, and adult mouse islet nuclear extracts as compared with Actin loading control (bottom). Arrowhead points to the Lhx1-specific signal, based on apparent size. Data are representative of at least 3 independent experiments. Pa, pancreas; St, stomach. ***P 0.001.
Isl1, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/isl1/product/Developmental Studies Hybridoma Bank
Average 97 stars, based on 1 article reviews
isl1 - by Bioz Stars, 2026-03
97/100 stars
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mouse anti islet1 2  (Developmental Studies Hybridoma Bank)
97
Developmental Studies Hybridoma Bank mouse anti islet1 2
Fig. 1. LIM homeobox 1 (Lhx1) is expressed in the pancreas. A: embryonic day (E)14.5 Lhx1-LacZ reporter mouse pancreas tissue staining demonstrates an active Lhx1 locus in the developing pancreas. Tissues were stained with X-gal (blue) and eosin (pink). Bottom: 40 magnification of E14.5 Lhx1-LacZ reporter activity in mouse pancreas, highlighted by black arrowheads B: immunofluorescence from 5-mo-old adult mouse pancreas tissue demonstrating Lhx1 (red) in insulin (green) co-expressing cells. C: co-immunofluorescence with 5-mo-old wild-type mouse pancreata indicates that Lhx1 (blue), <t>Islet-1</t> <t>(Isl1;</t> red), and LIM domain-binding protein 1 co-regulator (Ldb1; green) are co-expressed in islets. D: Lhx1 and Isl1 expression relative to Gapdh was assessed with TC3, Min6, and adult mouse islet cDNA (top); Western blotting analysis of relative Lhx1 protein levels in TC3, Min6, and adult mouse islet nuclear extracts as compared with Actin loading control (bottom). Arrowhead points to the Lhx1-specific signal, based on apparent size. Data are representative of at least 3 independent experiments. Pa, pancreas; St, stomach. ***P 0.001.
Mouse Anti Islet1 2, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti islet1 2/product/Developmental Studies Hybridoma Bank
Average 97 stars, based on 1 article reviews
mouse anti islet1 2 - by Bioz Stars, 2026-03
97/100 stars
  Buy from Supplier

Image Search Results


Fig. 1. LIM homeobox 1 (Lhx1) is expressed in the pancreas. A: embryonic day (E)14.5 Lhx1-LacZ reporter mouse pancreas tissue staining demonstrates an active Lhx1 locus in the developing pancreas. Tissues were stained with X-gal (blue) and eosin (pink). Bottom: 40 magnification of E14.5 Lhx1-LacZ reporter activity in mouse pancreas, highlighted by black arrowheads B: immunofluorescence from 5-mo-old adult mouse pancreas tissue demonstrating Lhx1 (red) in insulin (green) co-expressing cells. C: co-immunofluorescence with 5-mo-old wild-type mouse pancreata indicates that Lhx1 (blue), Islet-1 (Isl1; red), and LIM domain-binding protein 1 co-regulator (Ldb1; green) are co-expressed in islets. D: Lhx1 and Isl1 expression relative to Gapdh was assessed with TC3, Min6, and adult mouse islet cDNA (top); Western blotting analysis of relative Lhx1 protein levels in TC3, Min6, and adult mouse islet nuclear extracts as compared with Actin loading control (bottom). Arrowhead points to the Lhx1-specific signal, based on apparent size. Data are representative of at least 3 independent experiments. Pa, pancreas; St, stomach. ***P 0.001.

Journal: American journal of physiology. Endocrinology and metabolism

Article Title: The islet-expressed Lhx1 transcription factor interacts with Islet-1 and contributes to glucose homeostasis.

doi: 10.1152/ajpendo.00235.2018

Figure Lengend Snippet: Fig. 1. LIM homeobox 1 (Lhx1) is expressed in the pancreas. A: embryonic day (E)14.5 Lhx1-LacZ reporter mouse pancreas tissue staining demonstrates an active Lhx1 locus in the developing pancreas. Tissues were stained with X-gal (blue) and eosin (pink). Bottom: 40 magnification of E14.5 Lhx1-LacZ reporter activity in mouse pancreas, highlighted by black arrowheads B: immunofluorescence from 5-mo-old adult mouse pancreas tissue demonstrating Lhx1 (red) in insulin (green) co-expressing cells. C: co-immunofluorescence with 5-mo-old wild-type mouse pancreata indicates that Lhx1 (blue), Islet-1 (Isl1; red), and LIM domain-binding protein 1 co-regulator (Ldb1; green) are co-expressed in islets. D: Lhx1 and Isl1 expression relative to Gapdh was assessed with TC3, Min6, and adult mouse islet cDNA (top); Western blotting analysis of relative Lhx1 protein levels in TC3, Min6, and adult mouse islet nuclear extracts as compared with Actin loading control (bottom). Arrowhead points to the Lhx1-specific signal, based on apparent size. Data are representative of at least 3 independent experiments. Pa, pancreas; St, stomach. ***P 0.001.

Article Snippet: The membrane was blocked using 5% nonfat dry milk in 1% PBS-Tween for 1 h at room temperature, followed by overnight 4°C incubation with -Lhx1 (sc-515631, 1:1,000; Santa Cruz Biotechnology or 4F2, 1:1,000; DSHB), -Isl1 (39.4D5, 1:1,000; DSHB), and -Ldb1 (sc-11198X,1: 1,000; Santa Cruz Biotechnology).

Techniques: Staining, Activity Assay, Expressing, Binding Assay, Western Blot, Control

Fig. 2. LIM homeobox 1 (Lhx1) interacts with LIM domain-binding protein 1 co-regulator (Ldb1) and Islet-1 (Isl1) in -cell lines. A: co-immunoprecipita- tion (IP) using TC3 nuclear extracts. Endogenous Ldb1 (top left) or Isl1 (top right) IP recovers Lhx1 as compared with input positive control and species- matched IgG negative control. Reciprocal Lhx1 IP is also enriched for Ldb1 (bottom left) and Isl1 (bottom right); n 3. B: co-IP using primary mouse islet extracts (n 3). Endogenous Isl1 IP recovers Lhx1. C: Lhx1 does not enrich for Pdx1, MafA, Nkx6.1, or Pax6 in TC3 cells, suggesting binding specificity with Ldb1 and Isl1 (n 3). WB, Western blot.

Journal: American journal of physiology. Endocrinology and metabolism

Article Title: The islet-expressed Lhx1 transcription factor interacts with Islet-1 and contributes to glucose homeostasis.

doi: 10.1152/ajpendo.00235.2018

Figure Lengend Snippet: Fig. 2. LIM homeobox 1 (Lhx1) interacts with LIM domain-binding protein 1 co-regulator (Ldb1) and Islet-1 (Isl1) in -cell lines. A: co-immunoprecipita- tion (IP) using TC3 nuclear extracts. Endogenous Ldb1 (top left) or Isl1 (top right) IP recovers Lhx1 as compared with input positive control and species- matched IgG negative control. Reciprocal Lhx1 IP is also enriched for Ldb1 (bottom left) and Isl1 (bottom right); n 3. B: co-IP using primary mouse islet extracts (n 3). Endogenous Isl1 IP recovers Lhx1. C: Lhx1 does not enrich for Pdx1, MafA, Nkx6.1, or Pax6 in TC3 cells, suggesting binding specificity with Ldb1 and Isl1 (n 3). WB, Western blot.

Article Snippet: The membrane was blocked using 5% nonfat dry milk in 1% PBS-Tween for 1 h at room temperature, followed by overnight 4°C incubation with -Lhx1 (sc-515631, 1:1,000; Santa Cruz Biotechnology or 4F2, 1:1,000; DSHB), -Isl1 (39.4D5, 1:1,000; DSHB), and -Ldb1 (sc-11198X,1: 1,000; Santa Cruz Biotechnology).

Techniques: Binding Assay, Positive Control, Negative Control, Co-Immunoprecipitation Assay, Western Blot

Fig. 3. LIM homeobox 1 (Lhx1) directly regulates glucagon-like peptide 1 receptor (Glp1R) in -cell lines. A: schematic of con- served MafA 5= control domains (left) and chromatin immunoprecipitation (ChIP) anal- ysis of Lhx1 enriching for the MafA region 3 domain (right). B: schematic of Glp1R 5= domains that specify known Islet-1 (Isl1)- bound ChIP peaks (left) and ChIP analysis demonstrating Lhx1 enrichment of the Glp1R 5= region (right). Values are pre- sented as fold enrichment over normal mouse IgG (set to 1-fold) after normalizing to the inactive albumin promoter. C: West- ern blot for Lhx1 or actin loading control using Min6 cell extracts transfected with a scrambled control siRNA (siSCR) or Lhx1 siRNA (siLhx1) (top); densitometry of Lhx1 protein band intensity normalized to actin (bottom). D: relative quantification of key -cell mRNA levels after siLhx1 knockdown demonstrating significant reduction of Glp1R in Min6 -cells. mRNA levels were normal- ized to Gapdh and compared with scrambled siRNA control. All data are shown as means SE; n 4–8 independent experi- ments. *P 0.05; **P 0.01; ****P 0.0001.

Journal: American journal of physiology. Endocrinology and metabolism

Article Title: The islet-expressed Lhx1 transcription factor interacts with Islet-1 and contributes to glucose homeostasis.

doi: 10.1152/ajpendo.00235.2018

Figure Lengend Snippet: Fig. 3. LIM homeobox 1 (Lhx1) directly regulates glucagon-like peptide 1 receptor (Glp1R) in -cell lines. A: schematic of con- served MafA 5= control domains (left) and chromatin immunoprecipitation (ChIP) anal- ysis of Lhx1 enriching for the MafA region 3 domain (right). B: schematic of Glp1R 5= domains that specify known Islet-1 (Isl1)- bound ChIP peaks (left) and ChIP analysis demonstrating Lhx1 enrichment of the Glp1R 5= region (right). Values are pre- sented as fold enrichment over normal mouse IgG (set to 1-fold) after normalizing to the inactive albumin promoter. C: West- ern blot for Lhx1 or actin loading control using Min6 cell extracts transfected with a scrambled control siRNA (siSCR) or Lhx1 siRNA (siLhx1) (top); densitometry of Lhx1 protein band intensity normalized to actin (bottom). D: relative quantification of key -cell mRNA levels after siLhx1 knockdown demonstrating significant reduction of Glp1R in Min6 -cells. mRNA levels were normal- ized to Gapdh and compared with scrambled siRNA control. All data are shown as means SE; n 4–8 independent experi- ments. *P 0.05; **P 0.01; ****P 0.0001.

Article Snippet: The membrane was blocked using 5% nonfat dry milk in 1% PBS-Tween for 1 h at room temperature, followed by overnight 4°C incubation with -Lhx1 (sc-515631, 1:1,000; Santa Cruz Biotechnology or 4F2, 1:1,000; DSHB), -Isl1 (39.4D5, 1:1,000; DSHB), and -Ldb1 (sc-11198X,1: 1,000; Santa Cruz Biotechnology).

Techniques: Control, Chromatin Immunoprecipitation, Transfection, Knockdown